应用RT—PCR方法,体外扩增获得人IL—6、IL—10、IL—13和SCF膜外区蛋白质编码序列.又运用基因重组手段,将这些基因序列分别插入到表达性载体PBV220中,转化大肠肝菌DH5α,经过筛选和鉴定,获得了上述细胞因子膜外区cDNA克隆.SDS—PAGE和生物学活性测定证实:IL—6、IL—10和IL—13已在大肠肝菌中得到表达,尤其是IL—6获得了高效表达,其表达量约占菌体总蛋白质含量的30%左右.

TNF gene was transfected into murine LAK cells by retrovirus. Our results showed that TNF gene-transfected LAK cells secreted TNF more than normal LAK cells and control gehe-transfected LAK cells. The in vitro growth ability and cytotoxicity of TNF gene-t

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本文受国家自然科学基金(39421009)资助