以大剂量化疗后进行同基因骨髓移植小鼠为实验模型,动态观察了成纤维细胞介导的IL-3基因疗法对骨髓移植后小鼠免疫功能重建的作用。结果发现,IL-3基因疗法能显著加快骨髓细胞的淋巴细胞增殖反应的恢复,并能显著增强腹腔巨噬细胞的杀伤功能,提高巨噬细胞分泌IL-1、TNF及脾细胞分泌IL-2等细胞因子的水平,从而证明IL-3基因疗法对骨髓移植后机体免疫功能重建具有促恢复作用。

In the present study,the effects of fibroblast-mediated IL-3 gene therapy on immune reconstitution was observed after syngeneic bone marrow transplantation in the mice which had received high-dose chemotherapy. After the treatment,the proliferation of bone marrow cells stimulated by ConA, the cytotoxicity of macrophages, the levels of IL-1, TNF induced from macrophage and IL-2 induced from the splenocyte of experimental mice were augmented significantly. The results suggest that the fibroblast-mediated IL-3 gene therapy could be used to accelerate the process of immune reconstitution and reduce the complication after bone marrow transplantation.

国家自然科学基金，优秀中青年人才专项基金(39421009)