以重组的复制缺陷性腺病毒为载体将鼠IL-4基因转染G422小鼠脑胶质母细胞瘤细胞,观察其体外生物学特性和体内致瘤性的变化。结果显示:基因转染的肿瘤细胞有目的基因mRNA的表达,分泌高水平的mIL-4,和野生型G422细胞相比,其体外的细胞增殖能力无显著差异,而接种皮下后肿瘤生长延缓、小鼠的存活期延长。

Murine interleukin-4 gene(mIL-4) was transfected into mice glioblastoma cell line G422 by recombinant adenovirus vector. We detected mRNA transcription of target gene in gene modified tumor cells(G422-mIL4). High level of mouse IL-4 could be detected in the culture supernatant of G422-mIL4. When inoculated subcuatneously, the tumor growth of G422-mIL4 was significantly inhibited as compared to wild type G422 and LacZ gene modified G422( G422-LacZ) . The period of survival of mice inoculated with G422-mlL4 was significantly prolongated(p<0.01).

自然科学基金