细胞因子基因转染的瘤苗在体内具有明显的抗肿瘤作用,但逆转录病毒载体转染的肿瘤细胞作为瘤苗在制备及保存上仍然存在不足。本文采用编码人IL-2基因的重组痘苗病毒载体(rVV-IL-2)转染B16-F10小鼠黑色素瘤细胞,经超声破碎及高速离心,制成IL-2基因转染的肿瘤细胞破碎物(IL-2VBO),以此作为瘤苗,局部注射治疗荷瘤小鼠,结果明显抑制了小鼠肿瘤的生长,延长其存活期,外周血淋巴细胞对B16-F10细胞的杀伤活性明显增高,而对YAC-1细胞的杀伤活性则增加不明显,表明IL--2VBO诱导了机体对B16-F10特异性的免疫功能。由于IL-2VBO制备方便,易于保存,同时由于痘苗病毒本身可加强诱导出的免疫反应,因此可望作为一种新的瘤苗应用于肿瘤的主动性免疫治疗。

In this experiment, the murine melanoma cell B16-F10 oncolysates transfected by recombinant vaccinia viruses encoding human IL-2(IL-2VBO) were used as vaccine. When the tumor-bearing mice were treated by following injection of IL-2VBO into tumor site, the tumor growth was inhibited and the survival time prolonged. The PBL from IL-2VBO treated mice showed higher cytotoxicity to the wild type B16-F10 but not to YAC-1 cells than that from control groups. The results showed the active specific immunity was induced by the IL-2VBO. On accout of its stronger immuogenicity and some advantages in preparing and storing, the IL-2VBO might be used as a kind of effective vaccine in the cancer active specific immunotherapy.

国家自然科学基金