本文介绍一种改进的蛋白质聚丙烯酰胺凝胶电泳的银染方法,通过对常用的Merril等人银染方法一些步骤的修改,染色后背景颜色明显减低、灵敏度明显提高,与原法相比提高近6倍,可检出低至0.38ng的蛋白条带。

A new modification of Merril's silver staining of proteins in sodium dodecylsulfate polyacrylamide gels is introduced.The use of a prolonged period step after fixation and adjusting of oxidant incubation time considerably decrease the background, therefore increase the sensitivity. The detection limit is fivefold lower than original method.