本研究探讨了FN对LAK细胞活性的影响.Extracellular Matrix Protein(ECM)中的一种成分FN是基底膜、细胞间质及细胞表面的重要组成成分.本文发现FN能调节LAK细胞的功能.首先、LAK细胞和FN的粘付能力被增强.第二、经过与FN共同培养的LAK细胞活性比正常要高15～20%左右.FN对LAK细胞活性增强机理是通过激活LAK细胞表面的LFA-1的活性(不是LFA-1数量的增加)来增强LAK细胞和肿瘤细胞的结合功能、提高LAK细胞的杀伤肿瘤细胞的能力.本文的结果表明FN-LAK可能会改善肿瘤治疗效果、并间接提示肿瘤细胞表面及肿瘤组织中的FN成分的多少可直接影响LAK细胞的治疗效果.

In this study, we demonstrated that immobilized fibronectin (FN) enhanced LAK activity, and that the enhanced LAK activity was completely abrogated by an anti-VLA-5 monoclonal antibody and RGD peptide. Fresh -spleen cells expressed VLA-4, VLA-6 and vitronectin receptor, whereas VLA-5 was expressed only on the spleen cells activated with IL-2. LAK cells showed increased adhesion to immobilized FN compared with that to control BSA, and the increased adhesion of LAK cells to immobilized FN was inhibited by anti-VLA-5 monoclonal antibody. Conjugate-formation assay showed that the LAK cells cultured on immobilized FN bound to target cells more efficiently than the control LAK cells, and that anti-LFA-1 monoclonal antibody inhibited the LAK-target cell binding. Immobilized type IV collagen and laminin, as well as FN, enhanced LAK activity. All these results suggest that the interaction of inte-grins expressed on LAK cells with extracellular matrix proteins act as co-stimulator for the enhancement of LAK activity , and that anchorage is necessary for full activation of LAK cells.

R733.1

国家教委博士点(93011)基金资助