以PHA激活的外周血淋巴细胞增殖以及LAK细胞杀伤K562细胞两项指标来检测肿瘤细胞系MCF7、MDA-453、MKN-45、Hela以及3AO细胞培养上清液中免疫抑制因子的活性.实验结果发现:所有的肿瘤细胞培养上清液都能在不同程度上抑制PHA激活的淋巴细胞增殖.除了MDA-453细胞外,其它肿瘤细胞培养上清液亦能抑制LAK细胞的杀伤活性.因此,这些肿瘤细胞培养上清液中确实存在着免疫抑制因子.以肿瘤细胞分化诱导剂苯乙酸钠处理肿瘤细胞,其培养上清液对PHA激活的淋巴细胞增殖以及IAK细胞杀伤活性的抑制作用显著降低.因此,苯乙酸钠能够降低肿瘤细胞产生的免疫抑制因子.

The immunosuppressive effect of supernatant of the cultured tumor cells, including breast adenocarcinoma ( MCF-7, MDA-453 ), gastrocarcinoma ( MKN-45 ), cervical cancer ( Hela ) and ovarian cancer ( 3AO ), was studied by MTT assay. The results showed that the supernatant of tumor cell culture suppressed the proliferation of lymphocytes activated with PHA. The supernatant of cultured tumor cells, except that of cultured MDA-453 cells, also suppressed the cytotoxic activity of LAK cells. It is suggested that there are immunosuppressive factors in the supernatant of tumor cell culture. But the immunosuppressive effect of supernatant derived from the cultured tumor cells treated with sodium phenylacetate that is a noncytotoxic differentiation inducer on the proliferation of lymphocytes activated with PHA and the cytotoxic activity of LAK cells , was decreased significantly. It indicates that sodium phenylacetate could decrease the immunosuppressive factors derived from tumor cells.

R730.1

上海市高校博士点建设基金(No.03509000)赞助