[关键词]
[摘要]
为探讨性激素受体(Sex Hormone Receptor)和糖皮质激素受体(Glucocorticoid Receptor.GR)与肝细胞癌(Hepa- tocelular carcinoma.HCC)的关系及其意义,应用放射配体测定13例HCC患者及23例正常人外周血淋巴细胞雌激素受体(Es- trogen Receptor.ER)和雄激素受体(Androgen Receptor.AR)及外周血细胞GR水平.正常人ER和AR分别为616±106和687±109位点/细胞;GR为5360±1684位点/细胞.HCC患者ER和AR分别为968±120和2903±860位点/细胞.HCC伴肝硬化GR为3112±1130位点/细胞、不伴肝硬化为4536±1201位点/细胞.AR水平明显高于对照组,提示HCC可能为雄激素依赖性肿瘤;GR水平与肝硬化负相关,提示GR下降反映肝硬化的程度.
[Key word]
[Abstract]
A crucial role in eliciting anti-tumor immunity of costimulatory molecule CD80(B7-1) has been demonstrated by animal experimental studies.In this paper, CD80 expression in human tumor cell lines and EBV-transformed B cell was detected using RT-PCR and FACS methods. The results showed that CD80 expression of Raji and EBV-transformed B cell was positive but that of 3AO,MCF-7,MDA-453,MKN-45, Hela was negative. We have constructed retroviral vector CD80-pLN,transfected package cell PA317, screened high titer rctrovirus supernatant then infected human tumor cell lines and gained CD80 positive tumor cell clones. With pre-and post-CD80-transfected human breast carcinoma cell line MDA-453,the upregulated expression of ICAM-I, HLA class I molecule was observed, but the expression of HLA class II molecule wasn't changed.
[中图分类号]
R73－3
[基金项目]
上海市科委重点项目资助
