本研究采用HL-60白血病细胞系探讨了rhGM-CSF/IL-3融合蛋白对Ara-C介导的HL-60细胞凋亡的影响,结果表明,Ara-C与rhGM-CSF/IL-3联合应用处理HL-60比Ara-C单独作用更能显著提高寡核苷体DNA片段、抑制HL-60集落形成;rhGM-CSF/IL-3对Ara-C介导的HL-60细胞凋亡率的影响比不加rhGM-CSF/IL-3的对照组有明显提高,且比rhGM-CSF与rhIL-3联用作用更明显,但对正常人外周血白细胞作用较弱,提示可能对于rhGM-CSF/IL-3诱导缓解期白血病具有一定作用.

The effect of rhGM-CSF/IL-3 on apoptosis of Ara-C-induced myeloid leukemic cell line HL- 60 was investigated. The results indicated that treatment with rhGM-CSF/IL-3 in combination with Ara-C significantly inhibited the colony growth of HL-60 and enhanced the oligonucleosomal DNA fragmentation as compared with Ara-C alone. The Ara-C mediated apoptosis rates of HL-60 cells treated with rhGM-CSF/IL-3 fusion protein alone were markablely improved compared with treatment with rhIL-3 plus rhGM-CSF, it was noted that the Ara-C mediated of apoptosis normal peripheral white blood cells was less affected by rhGM-CSF/IL-3. It suggested that rhGM-CSF/IL-3 could be used as a possible curing drug during the phase of induced remission of leukemia.

R730.59 R394

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