人ｐ１６基因是最近发现的一种肿瘤抑制基因。ｐ１６蛋白作为一种细胞增殖的负调控因子，在恶性肿瘤发生发展中起重要作用。为了进一步探讨ｐ１６基因的抗肿瘤特性，我们通过运用分子克隆技术构建重组人ｐ１６基因表达载体（ｐｃＤＮＡ３－ｐ１６），并通过电穿孔方法将ｐ１６基因导入到人肺腺癌ＮＣＩ－Ｈ４６０细胞中，经Ｃ４１８筛选获得可稳定表达ｐ１６的人肺腺癌细胞，观察ｐ１６基因对人肺腺癌细胞周期的影响和细胞增殖的作用

p16 gene was a tumor supressor gene found recently. The p16 protein is a negative regulator of cell proliferation . Loss of normal p16 function is associated with the development of neoplasms. To detect antitumorigenic effect of p16 on human lung adenocarcinoma cells, we cloned a p16 cDNA into the pcDNA3 vector at the sites of BamHl and Xho I to gain a p16 gene recombinant expression vector plasmid. We then transferred the p16 gene recombinant plasmid into human lung adenocarcinoma cell line (NCI-H460) by electroporation method. After G418 selection we assessed cell growth properties and cell cycle pattern by flow cytometry to G418-resistant clones of NCI-H460-pl6 and NCI-H460-vect respectively. The results show that human p16 gene could suppress the phenotype of NCI-H460 cell line and p16 gene therapy plays a positive role in human lung adenocarcinoma treatment.

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