观察重组腺病毒介导人野生型p53,B7-1和GM-CSF基因在肺癌细胞中的表达及致凋亡效应。方法：以复制缺陷型重组腺病毒为载体，将人野生型p５３，Ｂ7-1和ＧＭ-ＣＳＦ基因导入肺癌细胞，分别以免疫组织化学法、流式细胞分析法和ELISA法检测了外源基因在肺癌细胞中的表达，通过苔盼蓝染色后计数活细胞数绘制细胞生长曲线，末端标记法检测细胞凋亡。结果：观察到重组腺病毒介导的多种外源基因均可在肺癌细胞中高效表达，并可明显抑制肺癌细胞增殖并诱导其凋亡。结论：以上工作为进一步开展肺癌的多基因治疗打下了基础。

To observe the expression of human wild type p53, B7 1 and GM CSF genes mediated by recombinant adenovirus and their effects of inducing apoptosis on lung cancer cells. Methods: Human wild type p53, B7 1 and GM CSF genes were transfected into lung cancer cells mediated by recombinant adenovirus. The expression products of these genes were detected by immunohistochemistry assay, flow cytometric analysis and ELISA. Cell growth assay was carried out by counting alive cells after trypan blue exclusion. Cell apoptosis was detected by TdT assay of DNA fragmentation. Results: The multi genes could be efficiently expressed in lung cancer cells mediated by recombinant adenovirus, which could suppress lung cancer cell growth and induce their apoptosis. Conclusion: These results suggest the feasibility of muti gene therapy for lung cancer.