构建肿瘤相关基因SNC90哺乳动物细胞表达型载体并进行转染研究。方法：肿瘤相关基因SNC90cDNA片断亚克隆至哺乳动物细胞表达型质粒pREP9中，并运用脂质体和电穿孔法将重组质粒pREP9-SNC90转入3株人大肠癌细胞中，用G418筛选抗性细胞克隆。结果：重组体pREP9-SNC90对SW1116，COLO205和SW620肠癌细胞株克隆形成均有一定的抑制作用， 抑制率分别为72.2%，74.2%和59.7%。结论：这提示肿瘤相关基因SNC90对肠癌细胞生长起负性调节作用。

To construct eukaryotic expression vector of tumor-associated gene SNC90 and transfect it into human colorectal cancer cell lines. Methods: A 1.5 kb tumor-associate gene SNC90 full length cDNA was inserted into a mammalian expression vector pREP9 to make recombinant vector pREP9-SNC90, which was then introduced into three kinds of colorectal cancer cell lines, SW1116, COLO205 and SW620, by lipofection or electroporation. The cells resistant to G418 drug were selected. Results: Cells transfected with pREP9-SNC90 showed fewer G418-resistant colonies than those transfected with void vector, the inhibitory rates are 72.2%, 74.2% and 59.7%, respectively. Conclusion: SNC90 may play a negative role in regulating growth of colorectal cancer cell.

* 本课题受国家自然基金（39170800，39340007）资助