研究IL-18基因修饰后的树突状细胞(DC)的表型和功能的变化。方法：以含IL-18基因的重组腺病毒体外转染小鼠骨髓来源的DC，ELISA检测IL-18分泌水平，以RT-PCR检测IL-18 mRNA表达，以FACS法分析其DC表型变化，采用-TdR检测其MLR的变化。结果: IL-18基因修饰的DC有效分泌IL-18，可检测到IL-18 mRNA的表达，分泌上清具有诱导T细胞产生IFN-γ的作用，IL-18基因修饰的DC同种异体MLR明显增强，且仅能被IL-18抗体部分阻断，表型分析表明，IL-18基因修饰后的Ia，B7.2，ICAM-1，CD40阳性的DC的比例明显增加。结论: IL-18基因修饰的DC可有效表达有功能的IL-18，部分表面分子表达上调，刺激同种异体T淋巴细胞增殖能力的提高，提示其抗原提呈功能增强。

To investigate the phenotypes and immune stimulating activity of IL-18 gene modified dendritic cells(DC). Methods: The expression of IL-18 mRNA was detected by RT-PCR in bone marrow DC from C57BL/6 mice after transfection with advenovirus vector containing IL-18 gene, and the IL-18 in their culture supernatant was determined by ELISA, also the induction of IFN-γ production by T cells with the culture supernatant of IL-18 gene modified DC was determined. The surface molecular expression and allogeneic MLR of IL-18 gene modified DC were investigated by FACS and ［3H］-TdR incorporation method respectively. Results： IL-18 gene modified DC could secret IL-18 effectively, IL-18 mRNA could be detected in IL-18 gene modified DC, and the IL-18 gene modified DC could induce T cells to produce IFN-γ. The allogeneic MLR activity of DC increased significantly after transfection with IL-18 gene, and the activity could only be partly blocked by IL-18 antibody. FACS analysis showed that some surface molecules such as Ia，B7.2，ICAM-1，CD40 of DC were upregulated significantly after transfection with IL-18 gene. Conclusion： IL-18 gene modified DC could secret IL-18 effectively with normal function such as induction of IFN-γ production from T cells. Some surface molecules on DC were upregulated and allogeneic MLR activity increased after transfection with IL-18 gene. The data suggested that IL-18 gene-modified DC might have enhanced antigen presenting capacity.

* 本课题受国家自然科学基金(39730420，397080173)资助