摘要 目的：采用淫羊藿甙icarrin(ICA,从朝鲜淫羊藿中提取的中药单体),作用于人急性早幼粒白血病细胞HL-60,较系统地对ICA诱导肿瘤细胞凋亡进行了观察，为ICA的开发应用提供实验依据。方法：采用电镜、流式细胞术、DNA片段化检测、RT-PCR等技术进行研究。结果： ICA在体外诱导HL-60细胞凋亡，呈典型的细胞凋亡形态学和生化特征，并具有时间剂量依赖性。ICA诱导肿瘤细胞凋亡的同时,下调相关基因bcl-2和c-myc基因mRNA和蛋白表达水平。结论： ICA体外诱导HL-60细胞凋亡，其机理可能与下调bcl-2和c-myc基因mRNA和蛋白表达水平密切相关。

Abstract Objective: To examine if human acute promyelocytic leukemia cells (HL-60) apoptosis of could be induced by icarrin(ICA, a mononer purified from Epimedium Koreanum Nakai). Methods: The cells undergoing apoptosis were determined by morphology, DNA gel electrophoresis and flow cytometry (FCM). The expression of apoptosis associated genes bcl-2 and c-myc were evaluated by FCM and semi-quantitative RT-PCR methods. Results: ICA induced apoptosis showed classical morphological and biochemistry features in human cancer cells in a time and dose dependent manner in vitro. While inducing apoptosis, ICA downregulated expression of apoptosis associated genes bcl-2 and c-myc. Conclusion: This study suggests that ICA induced apoptosis of HL-60 cells is associated with downregulation of bcl-2and c-myc genes mRNA and protein expression.