摘要 目的：通过对rhTNF-α生物活性方法的改进，建立了较规范的活性测定方法, 避免了人为判定的误差，提高了测定精确性。方法：本研究应用L929鼠结缔组织来源纤维母细胞系，参照国际标准品测定rhTNF-α效价。分别用Northern Dot Blot杂交检测及DNA电泳等方法，观察不同效价rhTNF-α对HL-60人白血病早幼粒细胞生长的影响。结果： rhTNF-α对HL-60细胞的生长抑制作用与时间、剂量正相关，核酸水平检测初步发现c-myc基因表达量降低，且rhTNF-α对DNA有损伤作用。结论： rhTNF-α的测定可用自动分析的方法进行。rhTNF-α抑制HL-60细胞生长，其机理不同于阿霉素。

Abstract Objective: Through the improvement on determination of rhTNF-α bioassay, we established the formal procedure. Methods: We choose L929 cell for rhTNF-α bioassay determination. To identify the influence of rhTNF-α on the growth of HL-60 cells, various concentration and effecting time were studied. Results: The results showed that the inhibition of rhTNF-α on HL-60 cells based on dose and time. Northern Dot Blot and DNA electrophoresis showed c-myc gene was surpressed, and DNA was damaged by rhTNF-α. Conclusion: Autometic calculation is responsible for bioassay of rhTNF-α. rhTNF-α could inhibit the proliferation of HL-60 cells and its mechanism is different from doxirubicin.