了解鱼精蛋白应用于血管内皮生长因子受体介导的靶向性非病毒载体的可行性。方法：利用GV1，GV2靶向性非病毒载体来比较多聚赖氨酸与鱼精蛋白对靶向性基因转移复合体携带DNA的能力及体外基因转移效率的影响。结果：在A375细胞中，鱼精蛋白与多聚赖氨酸参与形成的复合体基因导入率都为50%左右。在ABAE细胞中，鱼精蛋白参与形成的复合体基因导入率只有20%左右，而多聚赖氨酸可达70%左右。鱼精蛋白参与形成的复合体导入引起的基因表达在转染后第6天达到高峰，传代培养不影响基因的表达水平；多聚赖氨酸在转染后第7天达到高峰，传代培养使基因的导入率明显下降。结论:鱼精蛋白在血管内皮生长因子受体介导的基因转移系统中的应用具有一定的限制性。

Purpose to investigate the different in vitrofunction of targetable non-viral vector containing poly-L-lysine or protamine. Methods： Using GV1 and GV2 targetable non-viral vectors, the influences of the poly-L-lysine and protamine on in vitro gene transfer efficiency and the course of gene expression were observed. Results： β-galactosidase was expressed at intermediate level (50%) in A375 cells using a complex containing either protamine or poly-L-lysine. However, in case of ABAE cells, β-galactosidase expression level was low (20%) transferred with a complex containing protamine. On the contrary, β-galactosidase expression was at high level (70%) provided that protamine was replaced with poly-L-lysine. In addition, β-galactosidase activity reached the peak at the 6th day after transfection with the complex containing protamine. The expression was not altered with subsequent subcultures, at least for 3 passages. Using poly-L-lysine, the expression peak in A375 reached the peak at the 7th day after transfection, but the level declined along with subsequent passages of cells. Conclusion: The apllication of protamine in VEGF receptor mediated gene delivery system was limited.

R730.2

* 本课题受国家“863”高科技项目资助 (课题号：Z-20-01--01)