目的：研究HER2/neu原癌基因胞外区片段(ECD) DNA直接肌肉注射在小鼠体内表达，体液免疫状况，利用小鼠流产模型观察其体内效应。方法：克隆并构建了人及大鼠HER2/neu原癌基因胞外区片段真核表达载体pCDNA3-X, 采用肌肉直接注射的方法免疫小鼠，分析目的基因在小鼠体内的表达及其诱导的免疫应答。结果：在质粒DNA直接注射部位可检测到目的基因的表达， 并诱导了针对HER2/neu的抗体反应；同时诱发小鼠流产，使免疫鼠产仔数减少，可观察到流产结节形成。结论：通过基因免疫，可诱导针对HER2/neu原癌基因产物的有效免疫应答。

To induce an immune response by intramuscular immunization with HER2/neu oncogene extracellular domain (ECD) DNA and observe the effect in vivo by an abortion model in mice. Methods: Human and rat HER2/neu ECD gene were cloned and inserted into expressive vector pCDNA3. The DNA immunization, expression of HER2/neu ECD, antibody detection and abortion in immunized mice were studied. Results: HER2/neu ECD protein expressed on muscle cells in the injected site, specific antibody to HER2/neu was induced by DNA immunization. The average of the offspring per delivery from immunized mice was significantly lower than that from control group, and the abortive embryos were found in uterus from immunized mice. Conclusion: The results show that both human and rat HER2/neu ECD gene can induced effective immune response by DNA immunization.

R730.51 R977.21

* 国家自然科学基金资助（39870707）课题