探讨HER2/neu原癌基因胞外区片段DNA免疫诱导的特异性细胞免疫应答及其在体内的抗瘤效应。 方法： 克隆并构建人HER2/neu原癌基因及其胞外区片段表达载体，分别转染SP2/0细胞以制备杀伤靶细胞。将质粒DNA免疫小鼠，观察其诱导的细胞免疫应答，同时分析免疫动物体内抗瘤效应。 结果： 体外获得稳定表达HER2/neu基因的SP2/0靶细胞。目的基因DNA免疫后，免疫鼠脾细胞在体外可检测到特异性杀伤作用，体内肿瘤细胞接种后可发现肿瘤结节出现时间延迟，肿瘤生长缓慢。 结论： HER2/neu原癌基因胞外区片段DNA免疫可诱导出特异细胞免疫应答，并具有一定抗瘤效应。

To study the cellular immune response and the anti-tumor effects induced by intramuscular DNA immunization with HER2/neu extracellular domain gene. Methods: HER2/neu oncogene extracellular domain gene was isolated and inserted to pCDNA3 expression vector. The cellular immune response and its anti-tumor effects were analyzed after intramuscular DNA immunization. Results: A specific cytotoxic activity by spleen cells from HER2/neu ECD gene immunized mice was found in vitro. Tumor growth was inhibited after tumor challenge in vivo in immunized mice. Conclusion: Specific cellular immune response and anti-tumor effect were elicited by HER2/neu extracellular domain gene immunization.

R730.51

* 本课题由自然科学基金项目(39870707)资助