观察用白细胞介素2（interleukin-2,IL-2）基因修饰小鼠骨髓来源的树突状细胞（dendritic cells, DC）后，DC表达细胞因子和趋化因子的变化及DC体内免疫后，小鼠体内主要脏器中IL-2表达情况。探讨其对抗原提呈微环境的改善及其可能的机制。方法:用ELISA方法检测DC培养上清和小鼠血清及脏器中IL-2的含量，用RT-PCR方法检测DC中IL-2,IFN-γ,IL-12,GM-CSF,IL-4,IL-10,TNF-α,IL-1β,MIP-1β,MCP-1,RANTES和IP10等的表达，用FACS分析DC表面IL-2受体的变化。结果： 经IL-2基因修饰后，DC除了分泌高水平的IL-2外，尚能分泌一定水平的IFN-γ和IL-2，其表面表达的IL-2Rα密度增加。RT-PCR分析表明其表达多种细胞因子和趋化因子，用IL-2基因修饰的DC免疫小鼠后，血清和脏器中能检测到高水平的IL-2。结论： IL-2基因修饰DC，能促进DC表达IL-2受体，并以自分泌形式分泌高水平的IL-2，优化其抗原提呈的微环境，提示可增强DC的抗原提呈功能。

To investigate the effect of IL-2 gene modification on expression of cytokines and chemokines in dendritic cells (DCs) and IL-2 expression in mouse organs afterin vivoDC immunization and to determine the improvement of microenvironment of antigen presentation and its potential mechanism. Methods: The IL-2 levels in DC culture, mouse serum and organs after IL-2 gene-modified DC(DC-IL-2) injection in vivowere examined by ELISA. The mRNA expressions of cytokine and chemokines (IL-2, IFN-γ, IL-12, GM-CSF, IL-4, IL-10, TNF-α, IL-1β, MIP1β, MCP-1, RANTES and IP10) in DC-IL-2 were analyzed by RT-PCR. The expression of IL-2Rα on surface of DC-IL-2 was analyzed by FACS. Results: After IL-2 gene modification, DCs could secret high level of IL-2, IFN-γ and IL-12. The expression of IL-2Rα on the surface of DC-IL-2 was increased. mRNAs of various cytokines and chemokines were detected. The levels of IL-2 increased immediately in mouse serum, lung, spleen and liver after DC-IL-2 injection in vivo. Conclusion: The IL-2 gene modification could enhance the biologic activity of the DCs and the microenvironment of antigen presentation was optimized.

R730.4

* 本研究受国家自然科学基金（39730420，39825123）资助