[关键词]
[摘要]
探讨分叉双歧杆菌的完整肽聚糖对巨噬细胞功能的调节作用。方法: 以完整肽聚糖注射于裸鼠腹腔,用小鼠胸腺细胞增殖法、L929细胞体外杀伤法及Griess试剂分别测定裸鼠腹腔巨噬细胞产生的IL 1,TNF-α及NO的水平;同时也观察了巨噬细胞体外杀瘤活性的变化。结果: 完整肽聚糖注射组裸鼠腹腔巨噬细胞产生的IL-1, TNF-α及NO含量均显著高于对照组,体外杀瘤活性也明显增强(P<0.01)。结论: 分叉双歧杆菌的完整肽聚糖能激活巨噬细胞,增强其杀瘤活性,并使之分泌多量的细胞毒性效应分子。
[Key word]
[Abstract]
To explore the adjustment of whole peptidoglycans of bifidobacteria to macrophage functions. Methods: The level of interleukin 1, tumor necrosis factor α and nitric oxide (NO) produced by peritoneal macrophages of nude mice was detected by employing mouse thymocyte proliferation method killing L929 cell method in vitro and Griess reagent,after whole peptidoglycans were injected into the nude mice peritoneally. Simultaneously, tumoricidal activity of themacrophages in vitro was observed. Results: The content of interleukin 1, tumor necrosis factor α and nitric oxide (NO) secreted from peritoneal macrophages of nude mice was significantly higher in the whole peptidoglycan group when compared with the control group. Futhermore, the tumoricidal activity of the macrophages in vitro was markedly promoted (P<0.01). Conclusion: Whole peptidoglycans of bifidobacteria bifidum could activated macrophages to secrete a lot of cytotoxicity effectors,and elevate its tumoricidal activity.
[中图分类号]
R739.41
[基金项目]