研究能分泌人白细胞介素2的重组卡介苗（rBCG-IL-2）对小鼠腹腔巨噬细胞体外杀伤活性功能的改变，探讨rBCG-IL-2对宿主免疫功能的影响。方法：采用基因工程技术和电转化技术构建卡介苗，采用MTT法测定小鼠腹腔巨噬细胞体外的杀伤活性。结果： rBCG-IL-2组小鼠腹腔巨噬细胞杀伤活性明显大于rBCG组和对照组，且巨噬细胞的杀伤活性在一周内达到最大。结论： rBCG-IL-2能提高巨噬细胞的杀伤活性，是一种很有前景的生物制剂。

To investigate the effect of recombinant BCG secreting IL-2 (rBCG-IL-2) on peritoneal macrophages of mouse and inquire into the effect of rBCG-IL-2 on immunity of host. Methods: Gene engineering technology, electricity transference and inoculation tumor cell are adopted; MTT assay is used to determine tumoricidal effect of macrophages. Results: Tumoricidal effect of macrophages of rBCG-IL-2 groups was higher than those of the control groups and BCG groups, and the tumoricidal effect of macrophages could reach a high state in a week. Conclusion: rBCG-IL-2 can raise tumoricidal effect of macrophages, improve immunity of host and is a prospect biological preparation.

R730.3

* 本课题受国家自然科学基金（39870944），广东省自然科学基金博士启动基金（97424）资助