研究巨噬细胞游走抑制因子（MIF）基因修饰的瘤苗诱导抗肿瘤免疫反应的机理。方法： MIF基因修饰的FBL3瘤苗小鼠免疫后，观察腹腔巨噬细胞数量和功能的变化。结果： MIF基因修饰的瘤苗免疫后，小鼠腹腔巨噬细胞的数量，表面免疫相关分子的表达，以及TNF-α和NO的分泌水平增高，吞噬功能、杀伤活性和抗原提呈功能增强。结论： MIF基因修饰的瘤苗体内免疫后，其分泌的MIF显著增强了巨噬细胞的功能，被活化的巨噬细胞在瘤苗诱导机体抗肿瘤免疫中起重要的作用。

To investigate the mechanisms involved in the induction of antitumor immune responses by MIF gene-modified tumor vaccine. Methods: The changes of the number and the functions of macrophages from the mice immunized with MIF gene-modified FBL3 tumor vaccine were analysed. Results: The number， the expression of surface molecules associated with immune response, the secretion of TNF-α and NO, the phagocytosis, the cytotoxicity and the antigen presenting activity of peritoneal macrophages from the mice immunized with MIF gene-modified tumor vaccine were all enhanced. Conclusion: The functional enhancement of macrophages is related to MIF secreted by MIF gene-modified tumor vaccine in vivo and the activation of macrophage may play an important role in the antitumor immune responses induced by MIF gene-modified tumor vaccine.

*本项目受国家自然科学基金（39670280）资助