为了探讨TGF-β1及其受体在肿瘤发病中的作用。方法： 本文选取了30例原发性肝癌患者的癌组织和癌旁组织，用免疫组化及核酸狭缝杂交，图像分析仪半定量的方法，从基因转录和蛋白质表达水平分析TGF-β1及TGF-β1受体的含量。结果：在肿瘤发生的情况下，癌细胞，癌旁组织细胞均表达TGF-β1，后者分泌量明显高于前者，同时，癌旁有大量的TGF-β1受体表达，而癌细胞大部分为阴性。结论： TGF-β1受体的缺失，可能是肿瘤细胞逃避TGF-β1抑制生长作用的方式。

To study the role of TGF-β1 and its type Ⅱ receptor in pathogenesis of hepatocellular carcinoma. Methods: The TGF-β1 and its type Ⅱ receptor in the tumor and surrounding tissue in 30 cases with hepatocellular carcinoma were investigated. Results: Levels of protein and mRNA were detected by immuno-histochemistry, hybridization and semi-quantitative analysis. The results showed that TGF-β1 in the tumor and surrounding tissue was overexpressed, and the levels of latter was even higher. TGF-β1 type Ⅱ receptor expressed in surrounding tissue, but it could not be found in most tumor tissues. Conclusion: The lack of TGF-β receptor in the tumor cell may be the way of cancer cells escaping the TGF-β1 action of inhibited proliferation.

R730.51

国家自然科学基金!（39870763），国家教委跨世纪人才培养计划基金资助