探讨抗PML-RARα反义核酸对早幼粒白血病细胞生长、细胞周期、细胞凋亡的作用。方法：以NB4细胞株为研究对象；细胞计数采用台盼兰排除、计数板计数法；白血病细胞集落采用甲基纤维素半固体培养；流式细胞仪(FCM)用于检测细胞凋亡和细胞周期。结果：以60 μg/ml的终浓度处理细胞，抗PML-RARα融合部位反义核酸（FUAS）能明显抑制NB4细胞增殖及白血病细胞集落(AML CFU)形成，最大抑制率分别为46.8%，51.6%；GM-CSF能减弱FUAS的作用，FUAS作用时间越短，GM-CSF促AML-CFU增加越明显；FUAS处理第7，9天，出现明显的凋亡细胞群，凋亡细胞百分比分别为41.0%，34.2%；FUAS处理第5天S期下降，G2/M期升高,第7天G0/G1期明显升高，S期回升。结论：抗PML-RARα反义核酸具有抑制早幼粒白血病细胞增殖、诱导细胞凋亡的作用。

To investigate the effects of PML-RARα antisense on the growth，cell cycle and apoptosis of NB4 cells. Methods： Cell apoptosis and cell cycle were detected by flow cytometry, and leukemic colony forming unit was exammed by methylcellulose assays. Results： PML-RARα antisence （FUAS） could inhibited the growth, and formation of acute myelocytic leukemia colony forming unit （AML-CFU）in NB4 cells； GM-CSF could enhance AML-CFU formation and antagonized the effect of FUAS on AML-CFU formation inhibition. The shorter the time of incubation with FUAS was, the more markedly GM-CSF enhanced the AML-CFU formation. At 7 days, 9 days of treatment with FUAS percentage of apoptotic cells was 41.5%, 34.2% respectively. Reduction of S phase and increase of G2/M phase at 5 days, significant increase of G0/G1 phase with S phase at 7 days. Conclusion: PML-RARα antisence can inhibit the cell growth and induce cell apoptosis of NB4 cells.

R730.51 R392.11

* 本课题受国家自然科学基金（39590291）资助