探讨人膀胱癌多药耐药细胞凋亡诱导途径。方法：应用免疫细胞化学、原位DNA末端转移酶标记法、相差显微镜及电镜技术，观察BIU-87/ADM细胞表面Fas抗原的表达和抗APO-1单抗对该细胞存活的影响及其特征。结果： Fas抗原在BIU-87/ADM中有高表达，经抗APO-1单抗作用后，BIU-87/ADM细胞存活率明显降低，光镜及电镜下观察到凋亡细胞的形态学改变，原位DNA末端转移酶标记阳性。结论： 抗APO-1单抗能与肿瘤细胞表面的Fas抗原结合，触发人膀胱癌多药耐药细胞凋亡。

To explore the methods of inducing apoptosis in human bladder cancer multidrug resistance cells. Methods： Expression of Fas antigen was detected with immunocytochemistry on BIU-87/ADM cells, and the effect of anti-APO-1 antibody was examined with in situ DNA terminal deoxynucleotydyl transferase assay (TDT)，phase-contrast and electron microscope techniques. Results： We found that Fas antigen was highly expressed on BIU-87/ADM cell surface, and after treatment with anti-APO-1 antibody, survival rate of BIU-87/ADM cells was decreased, apoptotic morphological changes and TDT positive cells were found. Conclusion： These results suggested that anti-APO-1 antibody could induce apoptosis of BIU-87/ADM cells by combining Fas antigen.

R730.51

国家自然科学基金!（39670280）资助