目的：探讨融合自杀基因ｐＣＥＡｅｄ－ｔＫ在导入到肺癌细胞后，其体外旁观者效应的细胞差异性。方法：融合基因ｐＣＥＡｅｄ－ｔｋ提取后，脂质体介导的方法转染到６训肺癌细胞株（ＳＰＣＡ－１，ＧＬＣ－８２，Ａ５４９，ＳＣ，ＨＬＡＭＰ，Ｌ－７８），用ＭＴＴ法测定染前后对前体药物５－氟胞嘧啶（５－ＦＣ）和丙氧岛苷（ＣＣＶ）的敏感性和在体外的旁观者效应。结果：（１）脂质体介导找历历志染效应低低。（２）在和种已转染

Objective: To investigate the variation in vitro bystander effect among human lung cancer cell lines aller transfection of pCEAcd-tk. Methods: pCEAcd-tk was extracted and transfected to six human lung cancer cell lines. Their sensitivity to 5- fluorocytosine (5-FC) and ganciclovir (GCV) was measured by MTT assay. Their bystander effect was also detendned. Re- sults: 1. The efficiency of lipid mediated gene transfer was very low; 2. Two out of six human lung cancer cell lines(SPCA-1, A549) have a high sensitivity to 5-FC and GCV; 3. Bystander effect: Two out of six human cancer cell lines(SPCA-1,SC) have a good bystander effect, and one out of them(A549) has a intermediate bystander effect, but three out of them (GM-82, HIAMP and L-78)have a poor bystander effect. Conclusion: There are a variation in vitro bystander effect in six human lung cancer cell lines after transfected with pCEAcd-tk.

R734.205 R730.51

*卫生部科研基金(编号413653)资助项目