[关键词]
[摘要]
1997年O'Reilly从小鼠血管内皮细胞瘤中分离到一种新的20 kD具有特异抑制血管内皮细胞生长的抑制因子——Endostatin，它对牛毛细血管内皮细胞、牛肺主动脉内皮细胞有特异的抑制增殖作用，而对非血管内皮细胞系细胞如NIH3T3，A10平滑肌细胞等则无增殖抑制作用，实验证实大肠杆菌生产的复性及未复性的Endostatin及杆状病毒生产的重组Endostatin均有抑制鸡胚血管生成的作用，而且Endostatin没有象肿瘤化疗药物那样引发肿瘤产生抗药性，其作用机理尚待进一步研究。
[Key word]
[Abstract]
Objective To investigate the effects of PML-RARα antisense on the growth，cell cycle and apoptosis of NB4 cells. Methods Cell apoptosis and cell cycle were detected by flow cytometry, and leukemic colony forming unit was exammed by methylcellulose assays. Results PML-RARα antisence （FUAS） could inhibited the growth, and formation of acute myelocytic leukemia colony forming unit （AML-CFU）in NB4 cells； GM-CSF could enhance AML-CFU formation and antagonized the effect of FUAS on AML-CFU formation inhibition. The shorter the time of incubation with FUAS was, the more markedly GM-CSF enhanced the AML-CFU formation. At 7 days, 9 days of treatment with FUAS percentage of apoptotic cells was 41.5%, 34.2% respectively. Reduction of S phase and increase of G2/M phase at 5 days, significant increase of G0/G1 phase with S phase at 7 days. Conclusion PML-RARα antisence can inhibit the cell growth and induce cell apoptosis of NB4 cells.
[中图分类号]
R730.2
[基金项目]
国家自然科学基金!（39590291）资助
