通过转染HSP90β反义核酸重组子pcDNA-HSP90于4株人消化系肿瘤细胞系，以建立HSP90β蛋白低调肿瘤细胞系, 并研究HSP90β蛋白低调后细胞的生长情况。方法： 用Lipofectamine介导将pcDNA-HSP90转染人胃癌细胞系SGC7901、人胃癌多药耐药细胞系SGC7901/VCR、人肝癌细胞系HCC7402及人食道癌细胞系Ec109，用G418进行筛选，用RNA酶保护分析法鉴定HSP90β反义核酸的表达，用 Western blot 法检测HSP90β蛋白的表达。用细胞生长曲线研究基因转染细胞的生长情况。结果： pcDNA-HSP90转染细胞AH-SGC7901，AH-SGC7901/VCR，AH-HCC7402及AH-Ec109有HSP90β反义RNA表达, 这些细胞HSP90β蛋白表达低调。研究还发现这些细胞的生长受到不同程度的抑制，其中AH-SGC7901/VCR及AH-Ec109细胞受抑程度更明显。结论： pcDNA-HSP90转染细胞AH-SGC7901，AH-SGC7901/VCR，AH-HCC7402及AH-Ec109 HSP90β蛋白表达降低，细胞的生长受到不同程度的抑制。

To set up HSP90β Protein down-regulated digestive tumor cell lines and study the influence of HSP90β to tumor cells. ethods: pcDNA-HSP90 of HSP90β anti-sense RNA reconstructs was transfected into SGC7901 of human gastric cancer cell line, SGC7901/VCR of MDR-type human gastric cancer cell line, HCC7402 of human hepatic cancer cell line and Ec109 of human esophageal cancer cell line by lipofectamine. Positive clones were selected by G418. Antisense RNA of HSP90β of the positive clones was detected by RNase protection assay and the expression of HSP90β protein was assayed by Western blot. Cell growth was studied by cell growth curve. Results: pcDNA-HSP90 transfected cells were named AH-SGC7901, AH-SGC7901/VCR, AH-HCC7402 and AH-Ec109 respectively and there were antisense RNA of HSP90β in them. HSP90β protein in them was lower than in their parental cells. Cell growth was inhibited to different degrees, and the growth of AH-SGC7901/VCR and AH-Ec109 cells were most greatly inhibited. Conclusion: The expression of HSP90β protein in AH-SGC7901, AH-SGC7901/VCR, AH-HCC7402 and AH-Ec109 cell lines was decreased and the study also showed that decreasing the expression of HSP90β could inhibit tumor cell growth.

* 国家自然科学基金第39570806号及国家杰出青年基金第3952020号资助