探讨化疗的敏感性与细胞凋亡的关系，观察细胞凋亡抑制分子bcl-2在卵巢癌化疗抵抗中的作用。方法： 构建了逆转录病毒bcl-2表达载体pLXSN/bcl-2，应用lipofactin法建立转染bcl-2基因和转染空载体pLXSN的卵巢癌细胞系。MTT法观察细胞系抵抗药物的细胞毒作用。FACS 和DNA琼脂糖凝胶电泳观察bcl-2高表达细胞系在阿霉素诱导细胞凋亡中的变化。结果：转染bcl-2的卵巢癌细胞系OC3/ bcl-2稳定表达bcl-2分子并抵抗药物介导的细胞毒作用，明显提高细胞的存活率，与此同时也抑制细胞凋亡的发生。结论：化疗的敏感性与细胞凋亡的调节密切相关，凋亡抑制基因bcl-2在肿瘤耐药中起重要作用。

To investigate whether bcl-2 directly contributes to the development of drug resistance and apoptosis in ovarian cancer cell lines cells OC3. Methods: Retrovirus expression vector pLXSN-bcl-2 was constructed and was transfected to ovarian cancer cell line cells OC3 using Lipofectin, with empty vector pLXSN as a control. Bcl-2 expression of transfected cells was analyzed by FACS and Western blot and Adr-induced cytotoxicity was measured by MTT assay. Apoptosis was also detected by PI DNA staining and DNA Ladder analysis.]Results: pLXSN/bcl-2 transfected cells OC3/bcl-2 overexpressed bcl-2 and were resistant to Adr-induced cytotoxicity. The ability against Adr-inducedapoptosis was increased. Conclusion: bcl-2 may play an important role in the resistance to Adr-inducing apoptosis, thereby increasing resistance of OC3/bcl-2 cells to chemotherapy.

*本课题受中国博士后基金(98623)、自然科学基金(39870784)共同资助