[关键词]
[摘要]
了解造血生长因子的不同组合对人脐血CD34 +细胞的扩增效应及分化特性。方法： 采用人脐血单个核细胞(MNC)经rhIL-1，rhIL-3，rhIL-6，rhG-CSF，rhGM-CSF和rhSCF的不同组合进行体外扩增培养, 动态观察了不同细胞因子组合对有核细胞总数的扩增数量,应用流式细胞技术(FACS)动态分析了造血细胞的表面标志, 并对液态扩增后造血细胞的粒 巨噬集落形成率(CFU-GM)进行了动态观察。结果:经上述6种细胞因子培养20 d, 有核细胞总数增殖44倍, 液态扩增培养8 d后, CFU-GM比原代MNC增加14.74倍, 扩增18 d后, CFU-GM明显减少, 仅为原代MNC的6.42倍, 且集落小于前者。扩增培养至6～8 d时, CD34+细胞总数是原代MNC的127.79～196.40倍, 18 d时下降至101.51倍。结论： 外源性造血生长因子可使脐血CD34+细胞及CFU-GM产生明显的扩增效应。
[Key word]
[Abstract]
To explore the role of human hematopoietic growth factors (HGFs) in proliferation and differentiation of cord blood CD34 +cells. Methods: Human cord blood mononuclear cells (MNC) were cultured with different combinations of HGFs (including rhIL-1, rhIL-3, rhIL-6, rhG-CSF, rhGM-CSF and rhSCF). The expansion folds of MNC, the changes of cellular surface markers by FACS, and CFU-GM of hematopoietic cells were observed. Results: The total nucleated cells expanded by 44 folds after cultured with 6 cytokines for 20 days. The number of CFU-GM increased by 14.74 folds after cultured in liquid for 8 days, but decreased heavily after 18 days. The total number of CD34+ cells increased by 127.79～196.40 folds at 6 to 8 day, but decreased to 101.51 folds at 18 day. Conclusion: Human hematopoietic growth factors can increase the expansion of cord blood CD34+ cells and CFU-GM significantly.
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[基金项目]
* 国家自然科学基金资助项目(39970828)和山东省自然科学基金项目(Q99C07)
