黑色素瘤B16细胞胞浆HSP-抗原肽复合物（HAC）的制备、免疫原性的诱导及其抑瘤作用的研究。方法:采用Tris-HCl提取和Sephacryl S-200凝胶过滤制备B16细胞HAC，通过C57BL/6J小鼠体内诱导特异性CTL，再经体内、体外实验检测其抑瘤作用。结果:凝胶过滤获得的含60～97 kD蛋白的41,47和53管的HAC可以降低肿瘤发生率、延长肿瘤出现时间及降低小鼠死亡率。结论: B16 细胞胞浆中的60～97 kD的HAC具有免疫原性及抑瘤作用，为制备肿瘤疫苗提供了实验依据。

To investigate the anti-tumor efficiency of B16 melanoma HACs in vivo and in vitro. Methods: Tris-HCl extract and Sephacryl S-200 gel filtration were applied to prepare B16 HACs, and the cytoloxicity of specific CTL induced by HACs was tested. Results: The 41, 47 and 53 tube HACs obtained by gel filtration could decrease the tumor incidences, delay the time of tumor development and decrease the mortalitites of mice. Conclusion: 60～97 kD HACs from B16 melanoma cytosol have the activites of inhibiting tumor and could be used in effective anti-tumor therapy.

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