研究淫羊藿甙(ICA)对PG细胞分泌转化生长因子β ２(transforming growth factor β ２，TGFβ ２)的影响、ICA逆转TGFβ2介导的免疫抑制作用及其机制。方法: MTT法检测细胞增殖、杀伤活性，RT-PCR检测TGFβ 2、穿孔素，流式细胞术检测TGFβ ２、IL-２Ｒα水平。结果: ICA可抑制PG细胞中TGFβ 2的蛋白和mRNA表达,能够逆转受TGFβ 2抑制的LAK和CD3AK细胞的杀伤活性，并能部分恢复受TGFβ ２抑制的LAK细胞表面IL-2Rα表达和CD3AK细胞内穿孔素mRNA水平,以及CD3AK细胞的增殖活性。结论: 淫羊藿甙通过抑制肿瘤细胞免疫抑制因子TGFβ 2的产生,增强免疫效应细胞的杀伤活性等机制而发挥抗肿瘤作用。

To investigate the influence of Icarrin(ICA) on transforming growth factor β ２ (TGFβ ２) expression of PG cells and the mechanisms of reversion of TGFβ ２ mediated immunosuppression by ICA. Methods: Cell proliferation and cytotoxicity was assayed by MTT assay. mRNA level of TGFβ ２ and perforin was assayed by RT-PCR assay. Expression of TGFβ ２ and IL-2R α was analysed by folw cytometry assay. Results: ICA could inhibit the protein and mRNA expression of TGFβ ２ in PG cells. It could also reverse the erpressed cytotoxicity of LAK and CD３ＡＫ cells by TGFβ ２. The IL-2R α expression on LAK and perforin gene transcription and proliferation of CD3AK inhibited by TGFβ ２ were also pastly recovered. Conclusion: The anti-tumor activity of ICA is at least partly due to its capability of inhibiting TGFβ 2 expression in tumor cells and restoring the cytotoxicity of immunocompetent cells inhibited by TGFβ ２

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