增强人肝癌细胞的免疫原性以激活宿主免疫细胞识别杀伤相应的肝癌细胞。方法: 以脂质体介导小鼠H-2K b基因转染肝癌细胞株，并检测H-2K b抗原表达情况，然后用H 2K b基因转染后的肝癌细胞体外激活效应细胞杀伤活性，再进行裸鼠动物实验。结果: H-2Kb基因转染人肝癌细胞株HepG 2后，Southern印迹杂交结果显示，H-2K b基因整合于肿瘤细胞染色体中，RNA点杂交结果显示，H-2K bDNA已转录成mRNA。免疫组化及流式细胞仪检测显示， H-2K b抗原已在肝癌细胞胞膜上表达。转染后肝癌细胞在体外能强烈诱发效应细胞毒性，这种细胞毒性现象在裸鼠实验中得到进一步验证。结论: 异源MHC Ⅰ类基因H-2K b转染肝癌细胞后可增强其免疫原性并激活免疫效应细胞杀伤活性。

To strengthen the immunogenicity of hepatocarcinoma cells and activate immnological cells recognizing and killing the tumor cells. Methods： The murine MHC-Ⅰ gene H-2K b which can express immunologial rejection antigens was transfected into human hepatocarcinoma cells HepG 2 by liposome DNA mediated gene transfer. The transfection and transcription of H-2K b gene were detected by molecular hybridization techniques. The exogeous antigens expressed on the membrane of transfected tumor cells were detected with ABC immunohistochemical method and flow cytometer. ［ 3H］ release assays were used to detect the recognizing and killing effects of lymphocytes to HepG 2 cells transferred with murine H-2K b gene. The nude mice experiment was used to further verify CTL cells killing active. Results: Southern blot hybridization showed that the H-2K b gene was integrated into the chromosome of HepG 2 cells. The RNA dot blot hybridization showed that there was transcription of H-2K b DNA in the transfected tumor cells. ABC immunohistochemical method and flow cytometer detection showed that the murine H-2K b antigens were expressed on the membrane of HepG 2 cells. ［3H］ release assays showed that the cytotoxicyty to HepG 2 cells transfected with H-2Kb gene was obviously higher than that to control cells. The results demonstrated that the growth of hepatocarcinoma cells which were transferred with H-2Kb gene was obviously inhibited. Conclusion： The murine MHC-Ⅰ gene H-2K b could be transferred into the human hepatocarcinoma cells and expressed on the membrane of transferred cells. The HepG 2 cells transferred with H-2K b gene could induce human effective lymphocytes to recognize and kill these transferred tumor cells.