观察应用重组IFN α2a能否增强单纯疱疹病毒胸腺嘧啶核苷激酶基因 (HSV tk) /丙氧鸟苷 (GCV)系统对卵巢癌细胞系SKOV3的杀伤效应。方法 :利用逆转录病毒载体将潮霉素磷酸转移酶和HSV tk的融合基因 (hytk)导入SKOV3细胞 ,测定GCV对SKOV3/hytk细胞的杀伤作用和旁观者效应。观察IFN α2a与HSV tk/GCV协同作用对旁观者效应的影响 ,并且在联合应用GCV和IFN α2a后 ,进行细胞周期分析。结果 :GCV对SKOV3/hytk细胞有明显的杀伤作用 ,并且可以同时杀伤周围未转基因的细胞 ,联合应用IFN α2a与HSV tk/GCV可以明显增强旁观者效应 (P <0 .0 5 ) ,使处于S期的SKOV3/hytk细胞较对照组和使用单药组明显增多 (P <0 .0 1)。结论 :IFN α2a与HSV tk/GCV联合应用可以产生协同作用 ,增强HSV tk/GCV系统在体外对卵巢癌细胞系SKOV3的杀伤效应 ,具有良好的应用前景

Objective:Express and purify the recombant protein in prokaryotic system, preparing the mouse anti human angiostatin polyclony antibody.Methods: The cDNA of angiostatin was amplified by PCR and was subcloned into vector pQE-30, and transformed into the E.coli BL21(DE3). At last angiostatin was purified and used to immune the mouse for preparing polyclony antibody .Results: The recombinant clones were picked out by the methods of restrictional enzyme cut and sequence analysis. Recombinant angiostatin was highly expressed when the strain was induced with 1mmol/L IPTG. Angiostatin was successfully purified and the polyclony antibody was also successfully prepared. Conclusion: The recombinant protein and the polyclony antibody can be used in further studies.

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