构建重组人FN多肽的真核表达载体 ,研究体内表达对免疫细胞的趋化作用及抑制肿瘤生长的作用。方法 :采用重组DNA技术构建表达质粒 ;体内外进行基因转染 ;用Westernblot方法鉴定表达产物 ;肌肉组织切片与染色观察体内基因转染后的趋化作用 ;小鼠实体瘤模型研究基因转染抑制肿瘤生长的作用。结果 :将人FNcDNA 5′端非编码区及信号肽编码区、CH5 0多肽编码区cDNA、人FNcDNA的 3′端非编码区重组连接并插入pcDNA3.1质粒 ,构建出pCH5 10。以pCH5 10转染小鼠NIH3T3细胞 ,可以表达产生CH5 0多肽。肌肉内注射转染pCH5 10可对免疫细胞产生趋化作用 ,抑制实体肿瘤的生长。结论 :质粒pCH5 10可在细胞中及小鼠体内表达 ;体内表达可对免疫细胞产生趋化作用 ,并可抑制实体瘤生长

Objective: To investigate the correlation between the expression of vascular endothelial growth factor(VEGF) and angiogenesis in tumor. Methods: VEGF 165 sense and antisense gene recombinants were introduced into human gastric cancer cells, respectively. Then the growth of transfected cells in nude mice and the microvascular density and histological change were examined. Results: The growth rate of tumor in nude mice inoculated with sense VEGF cells was markedly higher than that in nude mice inoculated with antisense-VEGF cells. In histological examination, the microvascular density in tumor caused by sense-VEGF cells was greatly higher than that in tumor caused by antisense VEGF cells. Conclusion: As starting angiogenesis, VEGF might promote the growth of tumor, and the inhibition of VEGF production might prevent solid tumor from growing.

R735.2 R730.23

本课题受国家自然科学基金（39870763）、教育部跨世纪人才培养计划基金资助