探讨OSM对黑色素瘤体内生长的抑制作用及其在基因 放射治疗中的应用。方法 :用重组的人OSM基因表达质粒 (pO)和辐射诱导性OSM表达质粒 (pEO)转染小鼠B16黑色素瘤细胞 ,筛选OSM表达细胞 (pO 17和pEO 1) ,皮下接种C5 7BL/ 6小鼠 ,观察和比较相同生长期内瘤体重量及给予60 Coγ 线局部照射后肿瘤抑制率的变化。结果 :在未照射实验中 ,pO 17和pEO 1细胞接种 2 0d后的瘤体重量较对照组明显降低 ;在照射实验中 ,pEO 1细胞照射后 7d后瘤体重量的下降最为显著 ,肿瘤抑制率由未照射时的 33.8%提高至 48.1%。结论 :转基因分泌的OSM可对瘤细胞的体内生长产生抑制 ;射线可通过Egr 1R增强OSM表达的途径加剧肿瘤抑制 ,即基因与射线的双重抑瘤效果

Objective: To generate an adenovirus, Ad-CD, expressing green fluorescence protein and bacterial cytosine deaminase, and use it for tumor suicide gene therapy. Methods: CD gene was cloned into pAdTrack-CMV, pAdTrack-CMV-CD and pAdEasy-1 were homologous recominated in bacteria. 293 cells were transfected by the newly-recombinated plasmid pAd-CD and generated adenoviruses. LoVo cells were infected with the adenovirus, and the tumor-killing effect after 5-FC was administered.Results: pAdTrack-CMV-CD and the newly-recombinated plasmid pAd-CD were tested by restriction endonuclease digestions. Followed by GFP, Ad-CD were produced in 293 and LoVo cells infected by Ad-CD were killed after 5-FC was administered.Conclusion: Adenovirus plasmid homologous recombinant in bacteria is a simple method for adenovirus generation. In vitro adenoviral carried CD gene is efficient for tumor cell suicide gene therapy and has a bystand-effect, which is not depended on cell direct contact

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本课题受国家自然科学基金(39670825)资助