探讨阳离子脂质体介导的基因转染效率与肺癌细胞增殖特性的关系。方法 :采用pCMVβ报告基因质粒及自杀基因E .colicd的真核表达载体评价脂质体介导的基因转染效率。肺癌细胞增殖特性的检测包括绘制细胞生长曲线、计算细胞群体倍增时间、流式细胞仪测定肺癌细胞的细胞周期分布。结果 :肺癌细胞的类型对脂质介导的基因转染效率具有显著影响。细胞增殖速度越快 ,处于分裂期 (G2 /M)的细胞越多 ,则脂质介导的基因转染效率越高。结论 :脂质介导的基因转染对细胞增殖的依赖 ,提示由快速增殖细胞构成的组织更适合阳离子脂质体介导的基因治疗

Objective: To improve the preparation of adherent lymphokine-activated killer cells (A-LAK cells) and to study the synergistic anti-tumor effect of phenylacetate(PA) and A-LAK cells. Methods: A-LAK cells were obtained from peripheral blood mononuclear cells (PBMC) of patients with hepatocellular carcinoma (HCC) by using L-phenylalanine methyl ester (PME) to deplete immunosuppressive monocytes. The proliferation of SMMC7721 cell line treated with PA was studied. A-LAK cells were treated with the supernatant derived from SMMC7721 cells treated with PA previously and the changes of proliferation and anti-tumor activity of A-LAK cells were observed. Results: The expansion of A-LAK cells was significantly higher than that of non-adherent LAK (NA-LAK) as well as standard LAK cells. The growth of SMMC7721 cells was significantly suppressed by PA. The supernatant of cultured tumor cells intensely suppressed the proliferation and cytotoxic activity of A-LAK cells, but the suppressive effect of supernatant treated with PA previously was decreased. Conclusion: A-LAK could be simply prepared by using PME, and have the synergistic anti-tumor effect with phenylacetate.

R735.7

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