研究NDV长春株和四平株致细胞死亡的方式，方法：采用空斑试验、细胞抑制试验、琼脂糖电泳及TUNEL染色等方法进行研究。结果：NDV长春株和四平株对鸡胚成纤维细胞（CEF）、BHK、HeLa,Hep-2,HCT和OS－732等肿瘤细胞产生明显的病变，而对人的羊膜细胞（Wish细胞）无明显影响。结论：长春株对肿瘤细胞的抑制作用强于四平株，长春株对肿瘤细胞的抑制不存在量效关系，只有量适剂量才引起最强的抑制作用，长春株和四平株在体外引起细胞死亡的途径不完全一致，长春株导致细胞死亡以凋亡为主，而四平株导致细胞死亡以坏死为主。

Objective: To investigate the mode of cell death caused by NDV strain Changchun and NDV strain Siping. Methods: Plaque formation, cell suppression test, gel electrophoresis, and TUNEL assay were used after the cells were infected by NDV. Results: The apparently pathological changes were observed in chicken embryo fibroblasts, BHK, Hela, Hep 2, HCT and OS 732 tumor cells, but not in Wish cells. The higher suppressed effect on tumor cells was found in the NDV strain Changchun than that in the NDV strain Siping. There was no dose effect relationship between NDV and tumor cell suppression, only optimum dose NDV could cause maximal tumor cells inhibitory effect. Conclusion: The mode of cell death might be different after infection of NDV. The NDV strain Changchun killed tumor cells mainly through apoptosis, while the NDV strain Siping killed tumor cells mainly through necrosis.

国家“973”项目（G199011902）