探讨人类肝癌细胞系 972 4在不同免疫力小鼠中的生长特点及T细胞对癌细胞的排斥特点。方法 :体外培养 972 4 ,接种到不同免疫力小鼠 (正常BALB/c ,B细胞缺陷的CBA/N ,T细胞缺陷的BALB/c nu ,T细胞、B细胞缺陷的SICD小鼠及免疫重建的SCID小鼠 )中 ,观察其生长特性 ;测定小鼠脾细胞杀伤力流式细胞仪测定外周血CD4 +,CD8+的百分率。结果 :BALB/c小鼠和免疫重建的BALB/c PBL SCID不成瘤 ;CBA/N随接种数量和途径不同而有不同的表现 ;BALB/c nu ,SCID小鼠和免疫重建的CBA/N PBL SCID10 0 %成瘤。接种过癌细胞的BALB/c和CBA/N小鼠脾细胞对癌细胞杀伤力较强 ,免疫重建的SCID杀伤力较小。不同组别CD4 +百分率都下降 ,CD8+变化不大 ,CD4 +/CD8+比值下降。结论 :小鼠成癌率与T细胞的作用最为密切 ;T细胞在异种瘤移植排斥中起主要的特异性的免疫杀伤作用

Objective: To explore the charateristics of human hepatocarcinoma cells growing in various strains of mice with different immunity and T lymphocyte rejection to the hepatocarcinoma cells. Methods: The human hepatocarcinoma cells were inoculated into various strains of mice with different immunity (including BALB/c, CBA/N, BALB/c nu, SCID and BALB/c PBL SCID,CBA/N PBL SCID) and their growths were surveyed. Then the spleen cells were harvested to examine their direct cytotoxicity to the hepatocarcinoma cells.The percentage of CD4 and CD8 cell of peripheral blood from mice was assyed by FCM. Results: There was no carcinoma growth in BALB/c and BALB/c PBL SCID,but was 100% carcinoma growth in SICD, nude and CBA/N PBL SCID. The spleen cells in experimental groups from BALB/c and CBA/N mice displayed strong cytotoxicity to target cells and those of immunoreconstituted SCID generated a little lysis to target cells, but those of control group and nude, SCID mice generated no lysis to target cells.The percentage of CD4 from BALB/c and CBA/N mice in experimental groups decreased,but the percentage of CD8 changed not much while CD4 /CD8 ratio decreased.Couclusion: Whether the hepatocarcinoma cells grew in mice correlated strongly with T lymphocytes which were xenospecific cytotoxicity and played a main role of immune killing in rejection to xenografted tumor.