从噬菌体12肽库和环7肽库中，筛选能够与KDR分子有特异结合活性的小肽。方法：以KDR/IgGFc为靶分子筛选噬菌体12和肽7肽库，经过3轮筛选和竞争性洗脱后，由ELISA、细胞-ELISA和竞争结合实验，鉴定阳性噬菌体克隆并测序。结果：从40个噬菌体克隆中得到12个能特异性与靶分子结合的阳性克隆，其中，6个噬菌体克隆与靶分子有较强的结合力，6个结合力较弱。结论：测序结果表明，12条小肽的一级结构中没有发现共同模式。特异性与KDR结合的活性小肽，有望在临床上作为放化疗药物的导向肽，以提高药物的选择性和降低其毒副作用。

Objective: To isolate small molecular polypeptides which bind to KDR specifically using C7 and 12 peptide libraries. Methods: KDR/IgGFc was coated directly on plates, C7 and 12 peptide libraries are then applied, followed by vigorous washings in washing buffers to remove most non-specifically bound phage and to select specific phage particals by VEGF suspension and elution in acid buffers. The positive phage clones were detected by ELISA, cell-ELISA and competitive binding ELISA. Results: After three washes, 12 positive phage clones were selected and sequenced. Conclusion: A conserved peptide motif was not found in these sequences. The peptide binding to KDR specifically may be helpful for lead drug to improve selectivity and decrease the side effect of anti cancer drug in clinical treatment.