建立干细胞因子（stem cell factor,SCF)体外生物学活性测定方法，用于该制品生物学效价测定。方法：应用类原巨核细胞白血病细胞系的UT－7细胞株，比较不同细胞浓度，培养时间等条件下应用MTT染色的效果，确定最佳实验条件，并用国家参考品校正SCF效价，结果：UT－7细胞对SCF的反应存在量－效关系，并确定了最佳测定条件和剂量范围，效价测定重复性好，结果准确，客观，并用于重组SCF新生物制品的效价测定，结论：已建立的UT－7细胞依赖株测定SCF生物学活性的方法可用于SCF的生物学活性的常规评价。

To establish a sensitive and effective method for bioassay of stem cell factor (SCF) in vitro used for evaluation of biological potency of this products. Methods: Leukemia cell line UT-7 was used in the bioassay of rhSCF. The optimal test condition was determined, by comparing different results of MTT staining from different cell concentration and cell culture time. The potency of SCF was calibrated by the National Reference. Results: According to dose-response curve of SCF on UT-7 cell proliferation, the optimal reaction time and dose range were determined. Conclusion: The established method of using UT-7 dependent cell line to test the SCF bioactivity can be used in routine quality control of the biological potency of recombinant SCF.

2000年国家高技术应用部门发展项目(编号:计司高技函[2000]104号)