目的：研究E1B缺陷腺病毒d11520对鼻咽癌CNE-2细胞的杀伤作用及其作用机理。方法：用MTT法和细胞病变试验（CPE）测量d11520在体外对CNE-2细胞的抑制和杀伤作用。并通过测定病毒在CNE-2细胞中的复制产量和DAPI染色试验探讨其杀伤机理；瘤内注射d11520，观察其对CNE-2裸鼠移植瘤的治疗效果。用免疫织化方法检测肿瘤组织中病毒的复制。结果：体外实验结果显示：d11520能在CNE-2细胞中复制，导致明显的细胞病变，显著抑制CNE-2细胞的生长，在病变细胞中可见明显的核膨胀，瘤内注射d11520能明显抑制裸鼠移植瘤的生长，在肿瘤组织中可检测到病毒复制。结论：d11520能在CNE-2细胞中复制并使之裂解，从而在体内外有效地抑制和杀伤CNE-2细胞。

Objective: To explore antitumoral efficiency and mechanism of dl1520, an E1B-deleted adenovirus against nasopharyngeal carcinoma CNE-2 cells. Methods: Growth inhibition of dl1520 on CNE-2 cells was examined with MTT colorimetric assay and cytopathic effect (CPE) assay. The propagation of dl1520 in CNE-2 cells was measured with plaque assay on 293 cells, and changes of nuclei in CNE-2 cells infected with viruses were observed under a fluorescence microscope after DAPI staining. For in vivo study, athymic mice bearing CNE-2 nasopharyngeal carcinoma xenografts were administered intratumorally with dl1520, tumor growth was monitored twice a week, and virus replication in tumor tissues was examined by immunohistrochemistry. Results: in vitro, dl1520 replicated in CNE-2 cells and induced obvous CPE, so inhibited effectively the growth of CNE-2 cells. Distinct nucleus expansion, but not the characteristics of apoptosis, was found in CNE-2 cells infected with dl1520. Compared with control, dl1520 inhibited the growth of tumor xenografts in vivo, and viral replication was found on a large scale in tumors treated with dl1520. Conclusions: The E1B-deleted adenovirus dl1520 replicated effectively in CNE-2 cells and inhibited the growth of CNE-2 cells both in vitro and in vivo.

国家杰出青年基金 ( 3982 5 12 4)，广东省医学科学技术研究基金 (B19990 72 )，中山医科大学“2 11工程”重点学科建设基金 ( 9816 8)