目的： 探讨重组分泌型人PSP94(rsHPSP94)对雄激素非依赖性前列腺癌细胞株PC-3生长的作用和作用机制。方法： 采用噻唑蓝(MTT)法检测rsHPSP94对PC-3的抑制率。流式细胞仪检测细胞周期分布的变化。结果： rsHPSP94以剂量和时间依赖性抑制PC-3的增殖，流式细胞仪分析表明细胞增殖阻滞在G1期，并出现凋亡。结论： 本实验结果表明rsHPSP94通过使细胞阻滞在G1期抑制PC-3细胞的增殖

Objective: To investigate the effect of purified recombinant secreted human prostate secretory protein of 94 amino acids (rsHPSP94) on the growth of androgen-independent human prostate cancer cells (PC-3) and its potential mechanism of action. Methods:Cell growth inhibition rate was determined by MTT and cell cycle distribution was analysed by Flow cytometry(FCM) .Results: rsHPSP94, in a dose- and time-dependent manner, inhibited the growth of PC-3. Flow cytometric analysis showed that rsHPSP94 arrested the cell cycle in the G1-phase and induced the cell apoptosis after treatment for 48 h. Conclusion: These results suggest that rsHPSP94 can inhibit the growth of PC-3 by arresting the cell cycle in the G1-phase.

本课题受吉林省科技发展计划项目（N0.20001110）、 JICA(日本国际协力事业团)资助。