目的： 研究丁胱亚磺酰亚胺(BSO)增强D-氨基酸氧化酶（DAAO）/D-丙氨酸（D-Ala）自杀基因系统在白血病基因治疗中的作用。方法： 应用逆转录病毒转染技术获得稳定表达DAAO的高致瘤性K562e单克隆细胞KDfGC，用PCR、原位杂交对DAAO基因修饰的KDfGC进行鉴定。应用MTT法检测D-Ala对BSO预处理过的KDfGC细胞的杀伤作用。结果： PCR和原位杂交分析证明DAAO基因已整合至细胞基因组中，并在mRNA水平上表达。D-Ala作用24 h，KDfGC细胞的半数抑制浓度（C50）为10.21 mmol/L，KDfGC+BSO的IC50为7.92 mmol/L，两者的95％可信区间不重叠。结论： BSO可增强DAAO/DAla系统杀伤K652e细胞作用。

Objective: To investigate the effect of BSO on DAAO/D-Ala system killing K562e cells. Methods: KDfGC cell stably expressing DAAO was obtained by retrovirus transfection. The integration and expression of DAAO gene in KDfGC cells were identified by PCR and in situ hybridization. The killing effects of D-Ala on KDfGC cells treating with 1mmol/L BSO were observed. Results: PCR and in situ hybridization analysis confirmed integration of DAAO gene in positive clone and expression of it at mRNA level. The IC50 of KDfGC and KDfGC+BSO treating with D-Ala for 24 hours were 10.21 mmol/L and 7.92 mmol/L, respectively. The 95% confidence limits of them were different. Conclusion: BSO can enhance the killing effect of DAAO/DAla system on K652e cells.

本课题受国家自然科学基金（39870710）资助