目的：利用Pichia pastoris 菌株高效表达重组人内皮细胞生长抑制因子（rh-Endostatin）,并将其纯化至均质。方法： 将构建的菌种Pichia pastoris X33/pLW202扩增后接种至发酵培养基中，诱导表达后收获上清，将上清浓缩后经亲合层析、凝胶层析后得到高纯度rh-Endostatin。结果：从发酵上清中可得到60 mg/L高纯度的rh-Endostatin ,经HPLC检测纯度达98%以上。结论：在Pichia pastoris 表达系统中，实现了rh-Endostatin的高效分泌性表达。

Objective: To highly express rh-Endostatin from Pichia pastoris and purify it to homogeneity. Methods: Constructed Pichia pastoris X33/pLW202 was amplified and inoculated to ferment media. The supernatant of the strain was collected after induction. Through ultrafiltration,affinity and gel chromatography, rh-Endostatin with high purication was obtained. Results: 60mg/L rh-Endostatin was obstained from supernatant. HPLC showed its purity was above 98%. Conclusion: High level expression of secreted rh-Endostatin has been successfully achieved in Pichia pastoris expression system.

辽宁省科学技术厅（2001226001）基金资助