目的：观察HSP70-肿瘤肽介导的非MHC-Ⅰ限制性抗肿瘤免疫效应。方法：通过主动免疫和继承性免疫保护试验，观察从热休克小鼠肝癌HCaF细胞纯化的HSP70 肿瘤肽对HCaF（MHC-Ⅰ类分子表达阴性）的抗肿瘤免疫效果。结果：用源自HCaF细胞的HSP70-肿瘤肽主动免疫小鼠，可获得对HCaF细胞攻击的主动免疫保护效果，雌性组明显优于雄性组。转输HSP70-肿瘤肽免疫后抗HCaF细胞攻击的小鼠脾细胞，能产生良好的过继性免疫保护效果，可耐受HCaF细胞反复攻击，以1×10 7个活瘤细胞攻击亦未致瘤。转输HSP70-肿瘤肽体外致敏的脾细胞，也能使宿主产生一定的抗HCaF免疫效果。结论：从HCaF细胞纯化的HSP70-肿瘤肽可激发宿主产生非MHC-Ⅰ限制性抗肿瘤免疫保护效应，其强度可藉反复攻击而增强，并可通过免疫脾细胞继承性传递。

Objective: To observe effective of antitumor immunity elicited by heat shock protein70(HSP70)-associated peptide complexes isolated from hepatoma (HCaF), a tumor model of no MHC-Ⅰmolecule expression in mice. Methods: Specific active immunization and adoptive cellular immunization assay was adopted to observe the immunoprotective effect elicited by HSP70-associated peptide complexes purfied from the HCaF.Results: Mice immunized with HSP70-associated peptide complexes were protected from HCaF challenge. The immunoprotective effect in female mice was better than the male groups. Adoptively transferred immune spleen cells of mice which had been immunized with HSP70-associated peptide complexes could be elicited immunity against HCaF challenge, and the mice free of tumor can resist repeated challenge, the maximal challenge-quantities exceeded 1×10 7 HCaF cells. Mice immunized once with spleen cells pulsed by HSP70-associated peptide complexes could also be elicited certain immunity against HCaF challenge. Conclusions: HSP70-associated peptide complexes derived from the HCaF can elicit no-MHC-Ⅰ molecule restrictive immunoprotective effect against HCaF and could be continuously enhanced by repeated challenge with alive HCaF cells. Adoptively transferred immune spleen cells of mice which had been immunized with HSP70-associated peptide complexes could provide protection against HCaF cells challenge.

国家自然科学基金资助项目（39730440-Ⅱ）