目的：探讨反义寡核苷酸（ASODN）对内皮细胞表达E-selectin和ICAM-1的抑制及黏附肝癌细胞的影响。方法： 应用ASODN转染内皮细胞，通过流式细胞技术测定TNF α诱导后内皮细胞膜E selectin 和ICAM-1的表达，RT-PCR半定量测定E-selectin 和ICAM-1 mRNA的表达，细胞黏附实验测定肝癌细胞与内皮细胞的黏附率。结果： 裸ASODN和脂质体-ASODN均降低了内皮细胞E selectin 和ICAM-1分子及其mRNA的表达，后者尽管浓度低，作用更为明显；E-selectin的ASODN显著降低了内皮细胞黏附肝癌细胞的黏附率。结论： ASODN能竞争性地抑制内皮细胞黏附分子的表达，在一定程度上减少了肝癌细胞的黏附。

Objective: To explore the inhibition of E-selectin and ICAM-1 expression in endothelial cells and the effects on adhesion of endothelial cells to hepatocarcinoma cells by antisense oligodeoxynucleotide（ASODN）.Methods:With treatment of ASODN, the changes of E-selectin and ICAM-1 protein expression in endothelial cells induced by TNF-α were examined by flow cytometry, E-selectin and ICAM-1 mRNA expression by RT-PCR, and the adhesion rate of endothelial cells to hepatoma cells by cell adhesion experiment. Results: Both nude ASODN and liposome-ASODN apparently brought down the expression of E-selectin and ICAM-1 protein, as well as mRNA, and the latter worked better for all that lower dosage. E-selectin ASODN significantly decreased the adhesion rate of endothelial cells to hepatocarcinoma cells.Conclusion: These data demonstratel that ASODNs were capable of selectively inhibiting the expression of E-selectin and ICAM-1 in endothelial cells and thereby, to a certain extent, reducing the adhesion between endothelial cells and hepatocarcinoma cells.

福建省教育厅基金（K99054）资助