目的： 构建分泌独特型免疫球蛋白(idiotype, Id) 的BALB/c小鼠B细胞淋巴瘤细胞株，建立动物模型，为进一步开展Id 树突状细胞(dendritic cell, DC)疫苗的免疫治疗提供物质基础。 方法： 采用降植烷和不完全弗氏佐剂间隔致敏6～8周龄BALB/c小鼠，继而采用细胞融合技术、ELISA法、免疫荧光标记和流式细胞术(flow cytometry, FCM) 筛选获得分泌Id的阳性克隆；体内诱生腹水和体外无血清培养法制备Id，优球蛋白沉淀法结合凝胶Suphecray-200过滤纯化Id；建立荷瘤模型，从荷瘤小鼠骨髓前体细胞诱导DC。 结果：获得2株稳定分泌Id的BALB/c小鼠B细胞淋巴瘤细胞SB4和SB5，Id均为IgM/κ；成瘤率均为100%，荷瘤小鼠平均生存期为（30±4） d。 结论： SB4和SB5能持续稳定分泌非自身反应性Id，具有良好的成瘤性和高转移率。

Objective: To establish the secreting idiotype immunogloblin (Id) BALB/c mouse B cell lymphoma animal model for further study of Id-dendritic cells (DC) vaccines. Methods: BALB/c mouse B cell lymphoma cell lines were obtained by fusing SP2/0 cells with BALB/c mouse spleen cells after immunization with pristane and incomplete Freund s adjuvant (IFA) respectively. Enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FCM) were used to screen the secreting Id clones and analysis the phenotypes. Both mice ascites induction and serum-free culture medium X-VIVO 20 were used to produce the Id of SB4 and SB5. By using euglobulin precipitation and gel filtration on Suphecray-200 to purify the Id. To assess the ability of tumorigenicity, （4～5）×10 5 SB4/5 cells were injected subcutaneously in the right axillary or into abdominal cavity of 6~8 week BALB/c mice. DC were induced from bone marrow progenitor cells of B cell lymphoma bearing mice with recombinant murine GM-CSF and IL-4. Results: Two secreting none auto-reactive Id malignant B cell lymphoma cell lines, named as SB4 and SB5, were screened. Chromosomes study, DNA index, cell cycle proliferative indexes, cell generation time and mitotic index analyses all demonstrated SB4 and SB5 possessing the characteristics of malignant tumor cell lines. Both Id proteins belonged to IgM/κ. SB4 and SB5 cells grew progressively in syngeneic mice. Tumors could be touched in all mice and the average life-span was 30± 4 days and typical DC could be induced. Conclusion: BALB/c mouse B cell lymphoma SB4 and SB5 cell lines created by cell fusion techniques could secrete non-auto-reactive Id and had the characteristics of B cell lymphoma cells.

国家杰出青年基金项目（39625024）、国家自然科学基金项目（39870825）、江苏省科委自然基金项目（BI98100）资助