目的：探讨重构型caspase-3基因的表达对肿瘤细胞的凋亡诱导作用。方法：用重组PCR的方法获得大、小亚基顺序颠倒的重构型caspase-3基因并将其克隆入真核表达载体pcDNA3，转染乳腺癌细胞系SKBr3细胞，通过HE染色、流式细胞仪分析及免疫组化等方法观察其表达后对细胞的促凋亡活性，并将载体DNA直接注射荷瘤裸鼠研究其对肿瘤的抑制作用。结果：重构型caspase-3基因可在SKBr3细胞内表达，转染组较同期对照组出现明显的细胞死亡现象，pcDNA3- revcasp-3重组质粒直接注入对荷瘤裸鼠肿瘤生长有明显抑制作用。结论：重构型caspase-3基因表达可诱导SKBr3细胞凋亡。

Objective: To investigate the apoptosis induction effect due to the expression of reversed caspase-3 gene in SKBr3 cells.Methods: Reversed caspase-3 gene was gain by recombinant PCR and cloned into expression vector pcDNA3 to transfected SKBr3 cells. The expression and the apoptosis induction effect of reversed caspase-3 gene on SKBr3 cells were observed by HE and immunohistochemical staining. The cell cycle of transfected SKBr3 cells were analyzed by FCM. The tumor suppression effect of reversed caspase-3 gene was observed through administrating recombinant plasmids to BALB/c nude mouses bearing SKBr3 tumor.Results: Reversed caspase-3 gene can be expressed in SKBr3 stably. Reversed caspased-3 protein can induce obvious apoptosis in vitro and inhibit tumor growth in vivo.Conclusion: Reversed caspase-3 gene can effectively induce SKBr3 cells to apoptosis.

国家高技术研究发展计划（863计划）（2001AA217101）、国家杰出青年科学基金（399Z5036）及军队杰出中青年人才研究基金（98J009）资助