目的:研究ICA,PJM逆转肿瘤细胞恶性表型，提高免疫效应细胞识别和杀伤的作用和机制。方法: MTT法检测ICA,PJM对PG细胞增殖的影响以及对CD3AK杀伤敏感性的影响； RT-PCR法检测ICA,PJM对bcl-2和c-fos基因mRNA表达水平的影响； 流式细胞术检测细胞表面HLA-A,B,C抗原表达的变化以及c-fos,bcl-2蛋白表达水平的变化。结果: ICA,PJM对PG细胞有明显的增殖抑制作用，可以提高细胞表面HLA-A,B,C分子的表达和c-fos蛋白的表达，抑制bcl-2基因的表达，提高CD3AK细胞对PG细胞的杀伤活性。结论: ICA,PJM可以逆转肿瘤恶性表型，提高免疫效应细胞对肿瘤细胞的识别和杀伤。

Objective: To explore the mechanism and reversion of malignant phenotypes in a highly metastatic human lung tumor cell line. Methods: Suppression of ICA and PJM on PG cell line and the effects of PJM and ICA on PG cells sensitivity to lysis by CD3AK effectors cell was studied by MTT assay; the expression of bcl-2 and c-fos was studied by RT-PCR and flow cytometry, the expression of HLA-A,B,C in PG cells was examined by flow cytometry. Results: ICA and PJM inhibited the growth of PG obviously. ICA and PJM could significantly enhance the expression of HLA-ABC antigen and c-fos gene. ICA and PJM decreased the expression of bcl-2 and enhanced the the susceptibility of PG cells to lysis by CD3AK cells.Conclusion: ICA and PJM can reverse the malignant phenotypes of PG cells, enhance the recognition and killing effect of T cells.

山东省科技厅资助项目（971226206）